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Peptides And Proteins
Application scenarios cover new hot spots and valuable research fields, such as protein purification and detection, disease-related research, immunology and biochemistry research, scientific research peptides, medicinal peptides, etc., to meet the needs of researchers at different stages. We have a complete customer service system and technical team, each peptide product purified by HPLC, more stable quality, more timely delivery.
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Membrane Proteins

Membrane proteins

The KS-V Peptide team has more than 20 years of high-expression vector design, expression condition screening, membrane protein purification and rapid detection platforms for membrane protein targets based on eukaryotic expression systems.

 

 

Detailed Introduction of Membrane Protein

GPCR proteins

 

 


G protein-coupled receptors (GPCRs) are membrane proteins that regulate various cellular responses to external stimuli. It is involved in a variety of diseases and is an important drug target; more than 50% of modern protein drugs are related to GPCRs. As membrane proteins, GPCRs are very large, structurally flexible, and difficult to crystallize.

 

G protein-coupled receptors are one of the largest members of the receptor protein family. They play important biological functions in the process of cell signal transduction and are important drug targets. Extracellular drugs or ligands bind to different types of receptors, the receptors are activated, and the signals are transmitted to downstream effectors by activating G protein heterotrimers, through second messengers (cAMP or IP-one, etc.) Regulates the activity of membrane ion channels and nuclear transcription.

 

 

 

 

                                   Membrane proteins

 

 

 

GPCR target - completed target

 

 

Receptor Family (Human) Nomenclature G Protein Coupling Host Cell Line
Adrenoceptors Alpha 1A receptor Gq family HEK293T
Alpha 2A receptor Gi family HEK293T
Alpha 2B receptor Gi family HEK293T
Beta 1 receptor Gs family HEK293T
Beta 2 receptor Gs family HEK293T
Cannabinoid receptors CB1 receptor Gi/Go family HEK293T
CB2 receptor Gi/Go family HEK293T
Chemokine receptors CCR1 receptor Gi/Go family HEK293T
CCR2 receptor Gi/Go family HEK293T
CCR3 receptor Gi/Go family HEK293T
Somatostatin receptors SSTR1 receptor Gi/Go family HEK293T
SSTR2 receptor Gi/Go family HEK293T
SSTR3 receptor Gi/Go family HEK293T
SSTR4 receptor Gi/Go family HEK293T

 

   

Receptor Family (Human) Nomenclature G Protein Coupling Host Cell Line
Glucagon receptor family GHRH receptor Gs family HEK293T
glucagon receptor Gs family HEK293T
GLP-1 receptor Gs family HEK293T
GIP receptor Gs family HEK293T
Tachykinin receptors NK1 receptor Gs family HEK293T
NK2 receptor Gs family HEK293T
NK3 receptor Gq/G11 family HEK293T
Vasopressin and oxytocin receptors OT receptor Gq/G11 family HEK293T
Calcium-sensing receptor CaS receptor Gq/G11 family HEK293T
Endothelin receptors ETA receptor Gq/G11 family HEK293T
Opioid receptors δ receptor Gi/Go family HEK293T
κ receptor Gi/Go family HEK293T
μ receptor Gi/Go family HEK293T

 

 

                          

 

GPCR Cell Line Construction Services

 

● Cell line validation


1. mRNA integration
2. Cell membrane surface expression (specific tags)
3. Has functional activity

 

● Method development


1. Liposome cis-transfer
2. Lentiviral Infection-Stable Transfection

 

● Monoclonal cell screening


1. 3 functional cell lines
2. Validation of 2 blank cell lines

 

● GPCR cell bank
Further validation using molecular pharmacology and other methods

 

Fully autonomously constructed GPCR stably transfected cell line

 

Glucagon receptor family stable cell lines (GLP1R, GIPR, GCGR) have been established, and more GPCR target cell banks are being constructed in batches.

 

Advantages: The cell line construction cycle is 6-8 weeks, and the functional verification is two weeks.

 

 

                                         Membrane proteins

 

Ion channel


As an important target, ion channel is the second largest drug target after GPCR. It occupies an important position in the research and development of new drugs for the central nervous system and cardiovascular system, and is a rapidly growing hot spot in drug research and development. More than 400 genes are known to encode ion channels, and 300 ion channels have been identified. Among them, there are more than 100 druggable targets with known functions, many of which are proven therapeutic targets.

 

Among them, ligand-gated and voltage-gated ion channels are ranked third and fourth respectively among the top five gene targets of existing drugs. After G protein-coupled receptors (GPCRs) and the nuclear receptor family. At present, the hot areas of ion channel targets include pain (Nav1.7, ASIC, etc.), epilepsy (potassium channel) and cardiac safety evaluation (hERG), etc.

 

                    Membrane proteins

 

Ion Channel Targets - Targets Completed

 

 

 

 

Voltage gated ion channels
Voltage-gated potassium channels Kv1.1,Kv1.2,Kv1.3,Kv1.4,Kv1.5,KV11.1(HERG),Kv1.6,KCNE1,KCNQ1
Sodium channels NaV1.4,NaV1.5,NaV1.6,NaV1.7
hyperpolarization inward rectifying potassium channel KAT1
Ca2+-Activated potassium Channel KCa3.1
Acid sensitive ion channels ASIC1a,ASIC1b,ASIC2a,ASIC2b,ASIC3
Calcium channels CaV2.1(P/Q-type),CaV2.2 (N-type),CaV3.2 (T-type),CaV1.2 (L-type)
Chloride channel CLCN1, CTFR
Ligand gated ion channels
Ach nAchR α9,nAchR α10
GABA GABAA1-GABAA6 

 

 

Products
Peptides And Proteins
Application scenarios cover new hot spots and valuable research fields, such as protein purification and detection, disease-related research, immunology and biochemistry research, scientific research peptides, medicinal peptides, etc., to meet the needs of researchers at different stages. We have a complete customer service system and technical team, each peptide product purified by HPLC, more stable quality, more timely delivery.
Home >

products >

Membrane Proteins

Membrane proteins

The KS-V Peptide team has more than 20 years of high-expression vector design, expression condition screening, membrane protein purification and rapid detection platforms for membrane protein targets based on eukaryotic expression systems.

 

 

Detailed Introduction of Membrane Protein

GPCR proteins

 

 


G protein-coupled receptors (GPCRs) are membrane proteins that regulate various cellular responses to external stimuli. It is involved in a variety of diseases and is an important drug target; more than 50% of modern protein drugs are related to GPCRs. As membrane proteins, GPCRs are very large, structurally flexible, and difficult to crystallize.

 

G protein-coupled receptors are one of the largest members of the receptor protein family. They play important biological functions in the process of cell signal transduction and are important drug targets. Extracellular drugs or ligands bind to different types of receptors, the receptors are activated, and the signals are transmitted to downstream effectors by activating G protein heterotrimers, through second messengers (cAMP or IP-one, etc.) Regulates the activity of membrane ion channels and nuclear transcription.

 

 

 

 

                                   Membrane proteins

 

 

 

GPCR target - completed target

 

 

Receptor Family (Human) Nomenclature G Protein Coupling Host Cell Line
Adrenoceptors Alpha 1A receptor Gq family HEK293T
Alpha 2A receptor Gi family HEK293T
Alpha 2B receptor Gi family HEK293T
Beta 1 receptor Gs family HEK293T
Beta 2 receptor Gs family HEK293T
Cannabinoid receptors CB1 receptor Gi/Go family HEK293T
CB2 receptor Gi/Go family HEK293T
Chemokine receptors CCR1 receptor Gi/Go family HEK293T
CCR2 receptor Gi/Go family HEK293T
CCR3 receptor Gi/Go family HEK293T
Somatostatin receptors SSTR1 receptor Gi/Go family HEK293T
SSTR2 receptor Gi/Go family HEK293T
SSTR3 receptor Gi/Go family HEK293T
SSTR4 receptor Gi/Go family HEK293T

 

   

Receptor Family (Human) Nomenclature G Protein Coupling Host Cell Line
Glucagon receptor family GHRH receptor Gs family HEK293T
glucagon receptor Gs family HEK293T
GLP-1 receptor Gs family HEK293T
GIP receptor Gs family HEK293T
Tachykinin receptors NK1 receptor Gs family HEK293T
NK2 receptor Gs family HEK293T
NK3 receptor Gq/G11 family HEK293T
Vasopressin and oxytocin receptors OT receptor Gq/G11 family HEK293T
Calcium-sensing receptor CaS receptor Gq/G11 family HEK293T
Endothelin receptors ETA receptor Gq/G11 family HEK293T
Opioid receptors δ receptor Gi/Go family HEK293T
κ receptor Gi/Go family HEK293T
μ receptor Gi/Go family HEK293T

 

 

                          

 

GPCR Cell Line Construction Services

 

● Cell line validation


1. mRNA integration
2. Cell membrane surface expression (specific tags)
3. Has functional activity

 

● Method development


1. Liposome cis-transfer
2. Lentiviral Infection-Stable Transfection

 

● Monoclonal cell screening


1. 3 functional cell lines
2. Validation of 2 blank cell lines

 

● GPCR cell bank
Further validation using molecular pharmacology and other methods

 

Fully autonomously constructed GPCR stably transfected cell line

 

Glucagon receptor family stable cell lines (GLP1R, GIPR, GCGR) have been established, and more GPCR target cell banks are being constructed in batches.

 

Advantages: The cell line construction cycle is 6-8 weeks, and the functional verification is two weeks.

 

 

                                         Membrane proteins

 

Ion channel


As an important target, ion channel is the second largest drug target after GPCR. It occupies an important position in the research and development of new drugs for the central nervous system and cardiovascular system, and is a rapidly growing hot spot in drug research and development. More than 400 genes are known to encode ion channels, and 300 ion channels have been identified. Among them, there are more than 100 druggable targets with known functions, many of which are proven therapeutic targets.

 

Among them, ligand-gated and voltage-gated ion channels are ranked third and fourth respectively among the top five gene targets of existing drugs. After G protein-coupled receptors (GPCRs) and the nuclear receptor family. At present, the hot areas of ion channel targets include pain (Nav1.7, ASIC, etc.), epilepsy (potassium channel) and cardiac safety evaluation (hERG), etc.

 

                    Membrane proteins

 

Ion Channel Targets - Targets Completed

 

 

 

 

Voltage gated ion channels
Voltage-gated potassium channels Kv1.1,Kv1.2,Kv1.3,Kv1.4,Kv1.5,KV11.1(HERG),Kv1.6,KCNE1,KCNQ1
Sodium channels NaV1.4,NaV1.5,NaV1.6,NaV1.7
hyperpolarization inward rectifying potassium channel KAT1
Ca2+-Activated potassium Channel KCa3.1
Acid sensitive ion channels ASIC1a,ASIC1b,ASIC2a,ASIC2b,ASIC3
Calcium channels CaV2.1(P/Q-type),CaV2.2 (N-type),CaV3.2 (T-type),CaV1.2 (L-type)
Chloride channel CLCN1, CTFR
Ligand gated ion channels
Ach nAchR α9,nAchR α10
GABA GABAA1-GABAA6