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Structural Biology
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structural biology >

Cyro-EM Services

Cyro-EM Services

KS-V Peptide has an expert team of structural biologists with strong academic and industrial experience and can offer high-quality structural biology services to pharmaceuticals, biotechnology companies, and research institutions. Our team's expertise and state-of-the-art technology allow us to provide unparalleled services that meet the needs of our clients.

 

 

Cryo-electron microscopy (cryo-EM) is a revolutionary technology in the field of structural biology.Combining with 3D reconstruction techniques, it allows acquisition of near-atomic resolution protein structures by rapidly freezing biological samples and using electron beam irradiation. Cryo-EM avoids the need for protein crystallization and is ideal for investigating the structures of membrane proteins (such as ion channels, GPCRs, and transporters) and large protein complexes, which are often challenging to crystallize.

 

 

  • Cryo-EM (Cryogenic electron microscopy) platform

 

 

Why do you need cryo-EM?

 

 

Why choose our cryo-EM services?

 

  • Our cryo-EM platform has advanced microscopes including one Titan Krios and one Glacios.
  • We have an expert team with strong expertise in structural biology, protein science and computation.
  • We can offer one-stop protein structural biology services from gene synthesis to cryo-EM structure.
  • We have extensive experience in challenging proteins preparation such as membrane proteins, ion channel proteins, GPCRs, and many state-of-the-art techniques for obtaining and stabilizing protein complexes.

 

 

 

Cryo-EM Workflow

 

 

Our Service Guide

 

 

Service item Process description Timeline Deliverables
Protein preparation We can provide construct design, gene synthesis, protein expression, and protein purification based on your demands. Depends on samples Quality control data of purified proteins, such as SDS-PAGE, size-exclusion chromatography
Negative staining

Negative stain sample preparation and TEM examination

Assess sample homogeneity

1 week Sample quality report including 5-10 micrographs of negative stain sample

Negative stain image collection and 2D classification analysis

Further evaluate particle size and shape

2 weeks Particle analysis data including information on the size and shape of the particles
Sample vitrification

Rapid freezing sample using FEI Vitrobot system and vitrification optimization with different grids and blotting conditions.

Collect a small amount of data to assess the quality of the vitrified samples.

2-3 weeks Sample vitrification process report including information on the condition used and data including 2D images to evaluate the quality of the samples.
Data acquisition Data acquisition using Tian Krios 300 kV cryo-TEM 1-2 weeks We can provide raw datasets, processed data including 2D class averages, 3D density maps, and 3D structure models.
Data processing Data processing including motion correction, CTF estimation, particle picking, 2D classification, 3D reconstruction, model refinement and model validation Depends on the data quality and the analysis complexity.
Model building De novo modeling using high resolution density map

 

 

 

 

Products
Structural Biology
Home >

structural biology >

Cyro-EM Services

Cyro-EM Services

KS-V Peptide has an expert team of structural biologists with strong academic and industrial experience and can offer high-quality structural biology services to pharmaceuticals, biotechnology companies, and research institutions. Our team's expertise and state-of-the-art technology allow us to provide unparalleled services that meet the needs of our clients.

 

 

Cryo-electron microscopy (cryo-EM) is a revolutionary technology in the field of structural biology.Combining with 3D reconstruction techniques, it allows acquisition of near-atomic resolution protein structures by rapidly freezing biological samples and using electron beam irradiation. Cryo-EM avoids the need for protein crystallization and is ideal for investigating the structures of membrane proteins (such as ion channels, GPCRs, and transporters) and large protein complexes, which are often challenging to crystallize.

 

 

  • Cryo-EM (Cryogenic electron microscopy) platform

 

 

Why do you need cryo-EM?

 

 

Why choose our cryo-EM services?

 

  • Our cryo-EM platform has advanced microscopes including one Titan Krios and one Glacios.
  • We have an expert team with strong expertise in structural biology, protein science and computation.
  • We can offer one-stop protein structural biology services from gene synthesis to cryo-EM structure.
  • We have extensive experience in challenging proteins preparation such as membrane proteins, ion channel proteins, GPCRs, and many state-of-the-art techniques for obtaining and stabilizing protein complexes.

 

 

 

Cryo-EM Workflow

 

 

Our Service Guide

 

 

Service item Process description Timeline Deliverables
Protein preparation We can provide construct design, gene synthesis, protein expression, and protein purification based on your demands. Depends on samples Quality control data of purified proteins, such as SDS-PAGE, size-exclusion chromatography
Negative staining

Negative stain sample preparation and TEM examination

Assess sample homogeneity

1 week Sample quality report including 5-10 micrographs of negative stain sample

Negative stain image collection and 2D classification analysis

Further evaluate particle size and shape

2 weeks Particle analysis data including information on the size and shape of the particles
Sample vitrification

Rapid freezing sample using FEI Vitrobot system and vitrification optimization with different grids and blotting conditions.

Collect a small amount of data to assess the quality of the vitrified samples.

2-3 weeks Sample vitrification process report including information on the condition used and data including 2D images to evaluate the quality of the samples.
Data acquisition Data acquisition using Tian Krios 300 kV cryo-TEM 1-2 weeks We can provide raw datasets, processed data including 2D class averages, 3D density maps, and 3D structure models.
Data processing Data processing including motion correction, CTF estimation, particle picking, 2D classification, 3D reconstruction, model refinement and model validation Depends on the data quality and the analysis complexity.
Model building De novo modeling using high resolution density map